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M94A3293.TXT
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1994-10-25
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Document 3293
DOCN M94A3293
TI The role of the gag protein myristoylation in HIV-1 production.
DT 9412
AU Furuishi K; Oh-ishi H; Misumi S; Matsuoka H; Ikeda S; Hantani Y;
Ashizawa H; Shoji S; Dept. of Biochem., Fac. of Pharm. Sci., Kumamoto
Univ., Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):109 (abstract no. PA0055). Unique
Identifier : AIDSLINE ICA10/94369282
AB OBJECTIVE: The purpose of the studies is to clearly determine the
protein myristoylation of the gag protein in HIV-1 infected cell lines,
and to resolve the role of the gag protein myristoylation in virion
particle formation. METHODS: HIV-1 producing cell lines (CEM/LAV-1,
U937/IIIB, U937/RF, H9/MN, Molt-4/IIIB, U1, ACH-2) were maintained at 37
degrees C in RPMI-1640 medium (10% FCS) in 5% CO2. The N-myristoylation
of the gag protein was determined both by fluorography with [3H]myristic
acid and by western immunoblotting analyses with anti N-myristoyl-Gly
monoclonal antibody. The role of the N-myristoylation was resolved by
fluorography, immunochemical and paper technological methods with
N-myristoyl inhibitors or cross-linkers. RESULTS AND DISCUSSION: The
N-myristoylation of the gag protein in HIV-1 producing cell lines was
reconfirmed and its blockage by the myristoyl inhibitors resulted in
depression of the product of HIV-1 progeny virus. The cross-linked
proteins (p17gag-gp41 and p17gag-gp160) were clearly detected by western
immunoblotting analyses after cocultivation of CEM/LAV-1 in the presence
of cross-linking reagents. These results suggest that the N-myristoyl
moiety of the gag protein may be important for maturation of infectious
viruses.
DE Blotting, Western Cell Line Cross-Linking Reagents Gene Products,
env/METABOLISM Gene Products, gag/CHEMISTRY/*METABOLISM Human HIV
Antigens/METABOLISM HIV Envelope Protein gp41/METABOLISM HIV-1/*GROWTH
& DEVELOPMENT/*METABOLISM Myristic Acids/*METABOLISM Protein
Precursors/METABOLISM MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).